HMGB1 and malignant colon neoplasm: Following exposure to oxidizing saline solutions, we identified an upregulation of key ICD-markers, such as calreticulin (CRT), heat shock protein 70 (HSP70), and high-mobility-group-protein B1 (HMGB1) on the cell surface of CT26 colon-cancer cells (Fig. 7a,c,e) using flow cytometry.