Computerised reconstructions of immunostained sections map the extent of the neuronal damage (loss of MAP-2 or NeuN signal) and its spatial relation with post-stroke glial (GFAP for astrocytes, Iba1 for microglia, MBP-1 for oligodendrocytes) and vascular (CD31 for endothelial cells) reactions. Here, AIF1 is linked to stroke disorder.