To prove that CXCR4 plays a crucial role in colorectal cancer invasion through activation of RhoA/ROCK signaling in vivo, Western blot was performed to examine the expression of RhoA, p-FAK/FAK, ROCK1 as well as p-MLC/MLC in colonic tissues of AOM/DSS-induced CXCR4+/− mice and Apcmin/+ mice. The gene discussed is MLC1; the disease is colorectal cancer.