Conversely, negative PD-L1 assessments resulted in ORR of 46.2% (RNA-seq) and 37.9% (IHC) for melanoma, 11.9% (RNA-seq) and 13–14% (IHC) for NSCLC, and 8.3% (RNA-seq) and 12.5–14.6% (IHC) for RCC, with the latter two histologies’ ORR dependent on IHC cutoff and staining interpretation. The gene discussed is CD274; the disease is renal cell carcinoma.