Western blotting assay and qRT-PCR assays showed that NUSAP1 was upregulated in the seven fresh primary cervical cancer specimens compared with that in the paired normal samples, and NUSAP1 was over expressed in the eight cervical cancer cell lines compared with that in the human cervical immortalized squamous cell line (Ect1/E6E7) (Fig. 1d,e,g,h). This evidence concerns the gene NUSAP1 and cervical carcinoma.