Splenocytes from control and anti-CD4 Ab-treated mice were isolated on day 7 post-ZIKV infection, stimulated in vitro with three immunodominant class I-restricted CD8 epitopes (PrM169-177, E297-305, or NS52783-2792), and analyzed for the frequencies of CD8+ T cells producing IFNγ alone or and IFNγ and TNF. Here, IFNG is linked to Zika virus infectious disease.