TNF and infection: We observed a reduction in the frequency of splenic plasma cells and GC B cells (Fig 6D and 6E) in CD4-depleted mice but saw no effect on the CD8+ T cell response, as reflected by the frequency of IFNγ- and IFNγ+ TNF-producing cells after in vitro stimulation of splenocytes with E297-305 ZIKV epitope (Fig 6F), on day 7 post-infection.