Overall, these results suggest that, although CD4+ T cells are required for the differentiation of plasma and GC B cells and for the production of virus-specific IgG, they are not involved in viral clearance during primary ZIKV infection via either the intravenous (RO) or intrafootpad routes, which contrasts with the role of CD8+ T cells [22]. Here, CD4 is linked to Zika virus infectious disease.