Just like in the EPM also the NSF confirmed enhanced anxiety in 5-HT1A(-/-) knockout mice, time spent in the center of the arena (Wt = 27.32±3.81s, 1A-ko = 17.57±2.6s, p = 0.042) is significantly decreased and time till feeding showed a trend towards longer waiting times (Wt 118.56±19.16s, 1A-ko 146.69±36.67s, p = 0.772) (S1F and S1G Fig). Here, HTR1A is linked to Anxiety.