There was no labeling with NCC, pendrin, or NHE‐3 antibodies in renal cysts, indicating the lack of B‐intercalated cells, principal cells, distal convoluted tubule cells, proximal tubule cells or thick ascending limb of Henle cells in cyst epithelium adult Ren1cCreTsc1 mice. This evidence concerns the gene SLC12A3 and cystic kidney disease.