To dissect confounding factors associated with clinical sitosterolemia and assess the impact of β-sitosterol, campesterol, stigmasterol and cholesterol to cardiovascular endpoints, we developed a mouse model of phytosterolemia using C57BL/6 mice with Abcg5 and Abcg8 double knockout (DKO) to compare with C57BL/6 wildtype (WT) mice36. The gene discussed is ABCG8; the disease is sitosterolemia.