The OS tumours OS04 and OS03 (Fig. 4c, Supplementary Fig. S2a–e), and BZ28 and BZ35 (Fig. 5c, Supplementary Fig. S4a–e) that had the highest CD8A gene expression, also had high expression of PD-1, Tim-3, LAG-3 and CTLA4, indicative of a complex dysfunctional state of the CD8 + TILs in these tumours. This evidence concerns the gene LAG3 and neoplasm.