Since all OS tumours had CD8+ T-cell infiltration (of varying degrees) inferred from CD8A gene expression, we wanted to investigate the expression level of several T-cell inhibitory receptors PD-1, Tim-3, LAG-3, and CTLA4 using the RNA-seq data to assess the functional state of these CD8 + TILs. This evidence concerns the gene CTLA4 and neoplasm.