In this study, we identified the alternative transcript of NOTCH3 missing exon 16 (−exon 16) (Fig. 1, ENST00000601011.1) by human exon expression profiles and subsequently experimentally validated the expression of alternative splicing events using droplet digital PCR (ddPCR) in a primary DLBCL cohort (n = 75). Here, NOTCH3 is linked to diffuse large B-cell lymphoma.