CD8A and tropical spastic paraparesis: To demonstrate the accuracy and robustness of our TCR repertoire UMI-filtering based approach, we included 106 TCR-β repertoires from 20 HC, 14 HAM/TSP and 34 MS patients, technical replicates (HAM/TSP = 3), longitudinal samples (HAM/TSP = 4; HC = 4; MS = 2) and pairs of sorted CD4+ and CD8+ T-cell subsets from HC (N = 10), HAM/TSP patients (N = 11), and MS patients (N = 4).