To determine whether GLS1 affects the proliferation of colorectal cancer cells in vivo, we injected 2 × 106 of HT29 cells (NTC, shHIF-1α, or shGLS1 subclone) subcutaneously into the inguinal region of SCID mice (N = 6 mice in each group). The gene discussed is GLS; the disease is colorectal cancer.