Interestingly, we did find that only tumor cell lines isolated from primary tumors, with mutations in the APC gene (SW480, HT29, and DLD-1), were more susceptible to CBD, and cells that contained mutations in the β-catenin gene (HCT116 and LS174) were only moderately impacted by CBD where viability did not typically drop below 50% even at the highest concentrations tested. This evidence concerns the gene APC and neoplasm.