Consistent with the observations by Ad-SIRT6 infection, transfection of WT-SIRT6 decreased the cell surface area and BNP expression in NRCMs treated with or without PE (Figures 4A–C), downregulated the mRNA and protein expression of NFATc4 (Figures 4D,E), and recovered the repression of p-NFATc4 level and p-NFATc4/NFATc4 ratio caused by PE treatment (Figures 4E,F), without affecting CaN expression (Figure 4G). Here, NPPB is linked to infection.