In particular, we found that a CRC subtype characterized by high Tregs and low dendritic cells (DC) showed high expression levels of LY6G6D in concurrence with decreased expression levels of several immune checkpoint molecules such as CTLA-4 and PD1 [2, 3] (Fig. 2a and b Cluster 2, green). This evidence concerns the gene PDCD1 and colorectal carcinoma.