Plasma glucagon, measured at time 450 min, increased from 62.6 ± 3.3 to 87.9 ± 8.4 pg/mL with HP-LF, but did not change with LP-HF: 65.1 ± 4.4 vs. LP-HF: 70.6 ± 4.8 pg/mL, (p < 0.001 for overfeeding, p = 0.026 for protein/fat content, and p = 0.001 for overfeeding x protein/fat content). This evidence concerns the gene GCG and hydrops fetalis.