Western immunoblotting detection of GLI1 in C6 or U87 lineages revealed the major expression of this protein in nuclear extracts of these GBM cells, as shown in Figure 4A. Expression of GLI1 was analyzed in cells treated for 24 h with neuropeptides at 10−7 M, following or not a 1 μM pretreatment with H89 or IA, as shown in Figure 4B–D. Here, GLI1 is linked to glioblastoma.