In the present study, we have designed the experiments to compare the autophagy mechanism, including mitophagy and the PI3K-Akt/mTOR pathway (which is one of the modulators of the autophagy pathway) in vitro, using GBM cells derived from human (U-87MG), mouse (GL261), and rat (F98) origins, their respective rat glial tumor cells (C6-glioma), and mouse neuroblastoma cells (N2a cells) after treatment with Cur and/or SLCP. The gene discussed is AKT1; the disease is central nervous system cancer.