We specifically investigated known pathogenic variants and PTVs in MODY genes, where we found two rare variants that were high quality, definitely pathogenic, and strongly associated with diabetes (Table 2): a very rare stop-gain variant in GCK (MIM: 138079) (OR = 68, 95% CI [14, 328], p = 2 × 10-8), and a nonsynonymous variant (p.Arg114Trp) in HNF4A (MIM: 600281) (OR = 2.9, 95% CI [1.7, 5.0], p = 3 × 10-4). This evidence concerns the gene GCK and diabetes mellitus.