Ten weeks after inoculation, whereas macroscopically visible metastases in the lung were not observed due to a limited monitoring time, we found that the orthotopic tumor growth was similar between 231-vec and 231-Gab1OE group but the micro-invasion into adjacent normal tissues was enhanced in 231-Gab1OE group, as evidenced by a tissue H&E staining and by a reduced laminin IHC staining in tissues collected from 231-Gab1OE injected mice, respectively (Additional file 2: Figure S7 g-S7i). Here, LAMB2 is linked to neoplasm.