We found that the co-administration of vitamin D and probiotic for 12 weeks to women with PCOS had beneficial effects on mental health parameters, serum total testosterone, hirsutism, hs-CRP, plasma TAC, GSH and MDA levels, but did not affect serum SHBG, plasma NO levels, acne and alopecia. The gene discussed is SHBG; the disease is polycystic ovary syndrome.