Vitamin D and probiotic co-supplementation was associated with a significant reduction in total testosterone (β − 0.19 ng/mL; 95% CI, − 0.28, − 0.10; P < 0.001), hirsutism (β − 0.95; 95% CI, − 1.39, − 0.51; P < 0.001), hs-CRP (β − 0.67 mg/L; 95% CI, − 0.97, − 0.38; P < 0.001) and MDA levels (β − 0.25 μmol/L; 95% CI, − 0.40, − 0.10; P = 0.001), and a significant increase in TAC (β 82.81 mmol/L; 95% CI, 42.86, 122.75; P < 0.001) and GSH levels (β 40.42 μmol/L; 95% CI, 4.69, 76.19; P = 0.02) compared with the placebo. This evidence concerns the gene CRP and Hirsutism.