Moreover, total serum bile acids reach levels as high as 300 μmol/L in patients with cholestasis.5, 6 In line with our in vivo findings, neither the FXR agonist INT‐747, the TGR5 agonist INT‐777 nor the combination of both induced cortisol secretion in H295R cells (Figure S3), although both receptors being expressed in H295R cells (Figure S4), supporting the concept that the herein observed effects of bile acids are independent of FXR and TGR5. Here, GPBAR1 is linked to cholestasis.