Later on, Chakravarti et al. analyzed the expression of total PI3K, AKT, and S6K in 92 gliomas with different malignancy degree by western blot and did not find any difference in the total expression of PI3K, AKT, and S6K between GBM and non-GBM tumors. The gene discussed is AKT1; the disease is glioblastoma.