Similarly, Korkolopoulou et al. analyzed the expression of mTOR, S6K, and 4EBP1 phosphorylation in 111 tissue samples (grades II–IV) by immunohistochemistry and validated their analysis in 3 primary GBM cell cultures by western blotting analysis [50] (Table 1). The gene discussed is RPS6KB1; the disease is glioblastoma.