Likewise, the mice fed the mHFD for 24 weeks exhibited higher serum levels of the serum AST and ALT, greater steatosis and TG accumulation, higher levels of Mcp-1 and Tnf-α mRNA, and more severe fibrosis in the liver than the mCD-fed mice (Supplementary Figs 8 and 9). Here, TNF is linked to steatosis.