TNF and metabolic dysfunction-associated steatotic liver disease: The mRNA expression of Tnf-α, which is associated with lipid metabolism, hepatic inflammation and fibrosis in the progression of NAFLD/NASH19,20, was significantly increased in the BL6J mice by feeding an HFD, but there was little change in the Tnf-α expression in the BL6N mice (Fig. 6 and Supplementary Fig. 5).