INS and steatosis: In addition, because the transgenic expression of the Nnt gene in the BL6J mice rescues their impaired insulin secretion and glucose intolerant phonotype30, and the deletion of Nnt gene in the C57BL/6JUnib substrain aggravates HFD-induced steatosis and TG accumulation in the liver31, the Nnt mutation most likely influenced the increased accumulation of hepatic TG noted in the BL6J mice on an HFD in the present study.