In both HepG2 liver cancer cells and immortalized LO2 liver cells, our data revealed production of a 3-kb polyadenylated RNA transcript from the sense strand, but not the antisense strand, of the C/EBPβ enhancer (Fig. 2a and Supplementary Fig. 2), which is consistent with the features of unidirectional eRNA11. This evidence concerns the gene CEBPB and liver cancer.