CD4 and neoplasm: Importantly, the levels of total CD4+CD25+CD127–Foxp3+ Tregs within the viable tumor-infiltrating lymphocyte fraction, as well as the proportion of Foxp3hi aTregs, and Ki67+, Helios+ and CTLA4+ conventional CD4+CD25+CD127–Foxp3+ Tregs, were not related to the detection of a concomitant intratumoral HPV16-specific type 1 T cell response (Fig. 3a).