Figure S4. Gating strategy for Treg subpopulations. Figure S5. TbethiFoxp3+ Tregs express higher levels of helios, CTLA4 and Ki67. Figure S6. The levels of tumor-infiltrating Foxp3hiTbethi Tregs correlated with the levels of infiltrating CD4 + Tbet+ and CD8 + Tbet+ cells, as well as with levels of highly activated infiltrating CD4+ and CD8+ T cells. This evidence concerns the gene TBX21 and neoplasm.