The present study provides evidence for the chemosensitizing effect of Kaempferol to 5-FU chemotherapy of LS174-R cells by exerting synergistic inhibitory effect through blocking the production of ROS and modulating JAK/STAT3, MAPK, PI3K/AKT and NF-κB signaling pathway, involved in the progression and development of colorectal cancer. Here, STAT3 is linked to colorectal cancer.