APC and colorectal neoplasm: Western blotting of lysates derived from APC mutant COLO320DM colorectal tumour cells exposed to MSC2504877 indicated that MSC2504877 increased AXIN2 protein levels and decreased β-catenin levels (Fig. 1D), suppressed canonical Wnt signalling in SW480 cell line that stably expresses luciferase driven by a TCF-dependent promoter (Supplementary Fig. 1) and suppressed mRNA levels of two key Wnt-driven transcripts, RUNX2 and AXIN2, as assessed by qRT-PCR (Supplementary Fig. 2).