Using RT-PCR, we found that palbociclib exposure caused an upregulation of CCND2 mRNA levels (Fig. 6G), commensurate with the increase in Cyclin D2 protein levels seen earlier (Fig. 6C,D); this increase in CCND2 mRNA levels was reduced to basal levels when cells were also exposed to MSC2504877 (Fig. 6G), providing a mechanistic explanation for the combined tumour cell inhibitory effects of palbociclib and MSC2504877. This evidence concerns the gene CCND2 and neoplasm.