Next, the expression of CBX6 was assessed by qRT-PCR and by Western blotting using the antibody (Millipore 09-030) in a human non-tumorigenic epithelial cell line, MCF-10A, and two human breast adenocarcinoma cell lines, MCF-7 and MDA-MB-231. This evidence concerns the gene CBX6 and breast adenocarcinoma.