We selected MCF-10A immortalized mammary epithelial cells expressing a constitutively active version of Src (Src Y527F) as an appropriate system for characterization of the Src-regulated kinome, as these cells exhibit a transformed phenotype in culture and model the Src family kinase signaling network characteristic of basal/triple negative breast cancer (TNBC) cells20. This evidence concerns the gene SRC and triple-negative breast carcinoma.