To examine the possibility that CCL2 upregulates the genes related to mucus hypersecretion (MUC5AC and SLC26A4) and the epithelial cell-derived cytokines (IL-25 and IL-33) in airway epithelial cells, the human pulmonary mucoepidermoid carcinoma NCI-H292 cells were treated with or without 100 ng/ml CCL2 for 18 h. The gene discussed is MUC5AC; the disease is pulmonary mucoepidermoid carcinoma.