In an attempt to elucidate the mechanism(s) through which PDPN affects the EMT pathway in papillary thyroid cancer, we studied the expression of several proteins involved in the E/R/M and EMT pathways following PDPN silencing in TPC1 and BcPAP thyroid cancer cell lines, i.e. CD44, RhoA, Snail, Twist1, Vimentin, Paxillin and PTK2. The gene discussed is PXN; the disease is thyroid cancer.