Another possible reason for reduced early callus formation could be that when applied in high doses, baicalein leads to downregulation of BCL-2 and an increase in BAX and BIM, leading to apoptosis in fast-growing cells, which has been shown in osteosarcoma cells at high (75 μM), but not low, doses (50 μM) [68]. Here, BAX is linked to osteosarcoma.