PAK1 and breast carcinoma: To determine if PAK1-regulated disassembly is exclusive to breast cancer cells, PAK1 was also knocked down in the renal cell carcinoma cell line 786-0 (Supplemental Fig. 2F), revealing similar results in carcinoma of a different disease site, suggesting that invadopodia regulation by PAK1 is conserved across various cancer cell types (Supplemental Fig. 2G and H).