TWIST1 and neoplasm: Moreover, GFP+ tumor cells harvested from the metastasized tibias and mammary fat pads of mice on day 28 had significantly greater sphere formation ability (Fig. 1c, right panel) and expression of EMT genes, including N-cadherin, Twist, Snail1, and Vimentin, than those in circulation or injected into the mammary fat pads (Fig. 1d left panel).