Our data showed that the +SS (4.5 dyne/cm2), which may mimic the hydrodynamic SS in the blood flow in the arterial vasculature, can trigger transition of epithelial breast tumor cells into stem-like CSLCs/TICs by upregulating stemness factor along with EMT factors primarily through modulation of ROS and NO generation, accompanied by downregulation of the ERK and GSK3β pathway. Here, GSK3B is linked to breast neoplasm.