Then, we tried to silence KRT19 expression by transducing control shRNA (scramble) and KRT19 targeted shRNA (shKRT19) into both colon and breast cancer cell lines (i.e., HCT116, HT29, MDA-MB231, and MCF7 cells), and the knockdown effect was confirmed by RT-PCR and Western blot analyses (Figure 2a). The gene discussed is KRT19; the disease is breast carcinoma.