Under basal conditions, percentages of CD4+ and CD8+ subsets showed no difference in the ND and LT T1D patients versus control subjects (Fig 1A, Kruskal–Wallis one-way analysis of variance p = 0.5954; 1b, Kruskal–Wallis one-way analysis of variance p = 0.4756). This evidence concerns the gene CD4 and type 1 diabetes mellitus.