Immunolocalization of CKAP5 showed that while CKAP5 displayed as punctate structures throughout the cytoplasm in THLE2 cells (Figure 2A, yellow arrow) and SKBR3 breast cancer cells (Supplementary Figure 1A, yellow arrow), it was also found at the cell periphery where it co-localized with actin (Supplementary Figure 1A, white arrows) and particularly concentrated at the lamellipodia of THLE2 cells (Figure 2A, white arrows). This evidence concerns the gene CKAP5 and breast cancer.