Furthermore, compared to vehicle (0.1% DMSO), Co-CM containing GEN from the co-culture system significantly decreased the protein expression levels of the cancer stem cell surface markers CD44, CD133 (Fig. 2c) and the multipotent transcription factors Nanog and OCT4 (Fig. 2d) in SKOV3 cells in a dose-dependent manner. The gene discussed is POU5F1; the disease is cancer.