RNF31 and neoplasm: In contrast, our transcriptome data revealed that HOIP (Rnf31), HOIL-1 (Rbck1), and Sharpin, components of LUBAC, were more highly expressed in LSCC compared with LADC tumor cells, which was confirmed by quantitative PCR (qPCR) and immunoblotting (Fig. 4, A and B).