To further expand the studies on the anti-tumorigenic effects of increased SOX9 levels in SOX10 KD, we performed SOX9 KD in both SOX10 KD A375M and WM266–4 melanoma cells and compared the effects with SOX10 KD alone and control on cell proliferation, invasion and oncogenicity using alamarBlue, transwell and colony formation assays respectively. This evidence concerns the gene SOX10 and melanoma.