Furthermore, since we did not observe in vitro a direct effect of RCBTB1 on migratory capacities of sarcoma cells, this aspect should be examined in vivo, to understand if this negative result is due to the limitations of two-dimensional culture systems (absence of relevant microenvironment, no chemical or physical gradient, etc.)or if the link between RCBTB1 loss and metastasis mainly relies on adaptative capacities and selective advantage for cancer cells. The gene discussed is RCBTB1; the disease is sarcoma.