AFP and hepatocellular carcinoma: In addition, by using a wider gene panel that included the above genes as well as additional genes described as markers of proliferating and self-renewing liver cells,32 we observed that, while the heatmap from an unsupervised analysis shows that the HCCs from CCl4-treated mice cluster with cirrhotic livers (Figure 4B; Table S1), it should also be noted that a group of genes (Afp, Scd2, Gpc3, Prom1, Lpl, Ttf3, and Mcm2) exhibit an expression profile more similar to DEN-induced HCC than to cirrhotic livers, thus indicating a progression to cancer of CCl4-induced tumors.