Wnt/β‐Catenin pathway regulates the expression of many genes involved in apoptosis and GSK‐3β plays a key role in Wnt/β‐Catenin signalling pathway.31, 32 Another study has shown that TIPE2 could suppress progression and tumourigenesis of esophageal carcinoma via inhibition of the Wnt/β‐catenin pathway.19 Similarly, our results indicated that the expression levels of Wnt3a, p‐β‐Catenin, and p‐Gsk‐3β in the TIPE2 group were lower than those in the Mock group. This evidence concerns the gene GSK3B and carcinoma of esophagus.