Recent studies had found a correlation between Notch1 and PI3K/AKT/mTOR1 signaling pathways in T-ALL cells [47–52] Notch1 could increase the expression of growth factor receptors (IGF1R and IL-7R), and decrease the p53 and PTEN levels in T-ALL cells, which was beneficial to the activation of AKT [47–52] In this study, we found that the addition of the AKT specific inhibitor LY294002 effectively inhibited the activation of AKT, promoted the apoptosis, and inhibited the expression of Notch1 and HEY1, which implied that the link between Notch1 and AKT signaling pathways was complex. This evidence concerns the gene NOTCH1 and acute lymphoblastic leukemia.