In the 50 lymph node metastasis positive tissues, 86.0% of the cancer samples (43/50) showed increased expression level of ClC-3 protein (compared with the lymph node metastasis negative samples, 2.39-fold difference, p < 0.01), and 14.0% of the lymph node metastasis positive samples (7/50) did not show an elevated ClC-3 protein expression. Here, CLCN3 is linked to cancer.