To further evaluate expression characteristics of ClC-3 gene, we tested ClC-3 protein and mRNA expression levels of three representative cervical carcinoma cell lines including SiHa, HeLa, C-33A, normal cervical epithelial cell line H8 and normal keratinocyte cell line HaCaT via western blotting and quantitative real-time PCR. This evidence concerns the gene CLCN3 and cervical carcinoma.