Network approaches combining transcription factor, methylation, and miRNA analysis demonstrate that up to 74.8% of variation seen in gene expression in mesenchymal vs. other subtypes is explained by miRNAs.35 Similarly, an alternate analysis comparing the most differentially expressed miRNAs to each CRC subtype demonstrated that these miRNAs also defined the mesenchymal phenotype.36 Both studies also found that downregulation of miR-200 (and family members) contributed significantly to regulating EMT, matrix remodeling, and TNF signaling via NF-kB. This evidence concerns the gene TNF and colorectal carcinoma.