Interestingly, although the target genes differed among cell lines, the upregulation of NOTCH3 as well as its target genes was observed in the spheroids of other HCC cell lines including HLE, HuH6, and PLC/PRF/5, whereas the NOTCH3 pathway was unlikely activated in those of HLF and HepG2 cells (Figure S3C). This evidence concerns the gene NOTCH3 and hepatocellular carcinoma.