To validate whether the expression level of BCL2L1 is causative for insensitivity to E7107 in cells, we knocked down BCL2L1 in A549 and NCIH1568 lung cancer cells using inducible shRNA targeting independent BCL2L1 sequences different from the shRNAs used in the pooled screening (Fig. 1e). This evidence concerns the gene BCL2L1 and lung cancer.