In order to examine the sensitivity to splicing modulation of BCL2 family genes, we conducted whole-transcriptome RNA-seq for five cancer cell lines representing different lineages, including a melanoma cell line COLO829, an endometrial cancer cell line ESS1, a colorectal cancer cell line HCT116, a pancreatic adenocarcinoma cell line ASPC1, and NALM6. The gene discussed is BCL2; the disease is pancreatic adenocarcinoma.